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Statistical Optimization of Process Parameters by Central Composite Design (CCD) for an Enhanced Production of L-asparaginase by Myroides gitamensis BSH-3, a Novel Species
<p>Background: The present study focused on the production of L-asparaginase using Solid State Fermentation (SSF) by <em>Myroides gitamensis</em>. <br />
Methods: Initially, five significant parameters (Carbon source; Nitrogen source, temperature, pH and incubation period) were identified that affect the production process of L-asparaginase using Classical One Factor at a Time (OFAT) optimization. An optimized L-asparaginase specific activity obtained by OFAT was recorded as 85.7 <em>IU</em>. Central Composite Design (CCD) was also employed successively to optimize the multiple parameters at a time and their results were compared. <br />
Results: Maximum L-asparaginase enzyme specific activity obtained by CCD method was 295.6 <em>IU</em> under the hold values of carbon source (wheat bran) 12 <em>g/L</em>, nitrogen source (yeast extract) 7 <em>g/L</em>, temperature 37<sup><span style="font-size:10.8333px">o</span></sup><em>C</em>, pH=7.5 and incubation period 47 <em>hr</em>. Upon validation, the obtained results proved that there was a good relation existing between the experimental and the predicted model (p<0.05). L-asparaginase activity was enhanced in statistical method up to 3.4 folds compared to that of classical method. <br />
Conclusion: Utilization of wheat bran as a low cost carbon source in SSF for the production of L-asparaginase enzyme makes the process economical and in turn reduces the environmental pollution by biotransformation to commercially useful bio product.</p>
Biotransformation, Bran, Environmental pollution, Fermentation, L-asparaginase, Myroides gitamensis, Wheat
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https://www.ajmb.org/En/Article.aspx?id=10355
https://www.ajmb.org/PDF/En/FullText/10355.pdf
VSSLTalluriDepartment of Biotechnology, University of Chemistry and Technology Prague, Technicka 5, Czech Republic, CZ 16628 Prague11395
SriLankaDepartment of Biotechnology, GITAM Institute of Science, GITAM University, Visakhapatnam- 530 045, Andhra Pradesh, India11396
SVRajagopalDepartment of Biotechnology, GITAM Institute of Science, GITAM University, Visakhapatnam- 530 045, Andhra Pradesh, India11397