Avicenna J Med Biotech arij002 Avicenna Journal of Medical Biotechnology 2008-2835 2008-4625 Avicenna Research Institute ajmb333 Rapid and Simple Detection of <i>Escherichia coli</i> by Loop-Mediated Isothermal Amplification Assay in Urine Specimens RamezaniReihanehKardoost PariziZahraGhorbanmehrNassimMirshafieeHamidehDepartment of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran 10 4 269 272 16 5 2017 28 8 2017

<p>Background: To improve urinary tract infection detection, we evaluated the specificity and sensitivity of Loop-mediated isothermal Amplification Method (LAMP) for detection of the <em>Eschericia coli (E. coli)</em> in urine samples, for the first time.&nbsp;<br /> Methods: Primers were designed to target the malB gene of <em>Escherichia coli.</em> LAMP assay was performed on urine specimens collected from patients with urinary tract infection symptoms.<br /> Results: As expected, LAMP was more specific and sensitive than direct microscopic tests. LAMP assay showed the best detection limit of DNA copies with 1.02 copies.<br /> Conclusion: LAMP method offers several advantages in terms of sensitivity, rapidness and simplicity for detection of <em>E. coli</em> infection in urine samples. The LAMP method would be highly suitable for the early detection of the UTIs and also comfort quick diagnosis of UTI in clinical laboratories with limited equipment.</p>