Avicenna J Med Biotech arij002 Avicenna Journal of Medical Biotechnology 2008-2835 2008-4625 Avicenna Research Institute ajmb271 Efficient Media for High Lipase Production: One Variable at a Time Approach SoleymaniSafouraNanobiotechnology Research Center, Avicenna Research Institute (ACECR), Tehran, IranAlizadehHouriDepartment of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, IranMohammadianHosseinDepartment of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, IranRabbaniEmadDepartment of Molecular Medicine, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, IranMoazenFatemehReproductive Biotechnology Research Center, Avicenna Research Institute (ACECR), Tehran, IranMirMohammad SadeghiHamidImmunology Research Center (IRC), Tabriz University of Medical Science, Tabriz, IranSamsam ShariatZiaedinDepartment of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, IranEtemadifarZahraDepartment of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, IranRabbaniMohammedReproductive Biotechnology Research Center, Avicenna Research Institute (ACECR), Tehran, Iran 9 2 82 86 12 3 2016 25 6 2016

<p>Background: Lipase enzymes have applications in a wide range of industries. A crucial determining factor of industrial prices of these enzymes is the culture media composition that is constantly under review by researchers. In this work, for maximum lipase production by <em>Bacillus sp. ZR-5</em>, culture media compositions were optimized using &quot;one variable at a time&quot; strategy.<br /> Methods: For this purpose, the culture medium parameters such as low and high cost carbon and nitrogen sources, substrates and incubation times were evaluated.<br /> Results: Maximum lipase activity was achieved after 24<em> hr</em> of incubation with 1.5% of glucose syrup (1600&plusmn;69.1 <em>u/mg</em>), 1% of fish powder (1238&plusmn;36.7 <em>u/mg</em>) and olive oil (1407&plusmn;2.1 <em>u/mg</em>) as low cost carbon and nitrogen sources and substrate, respectively.<br /> Conclusion: Our results show a significant increase in lipase activity with usage of low cost sources; this could help in reducing the media prices for industrial application of lipase enzyme.</p>