Avicenna J Med Biotech arij002 Avicenna Journal of Medical Biotechnology 2008-2835 2008-4625 Avicenna Research Institute ajmb253 Novel Combinations of Synthesized ZnO NPs and Ceftazidime: Evaluation of their Activity Against Standards and New Clinically Isolated Pseudomonas aeruginosa IsaeiElhamDepartment of Pharmaceutical Biotechnology and Isfahan Pharmaceutical Research Center, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, IranMansouriShahlaDepartment of Pharmaceutical Biotechnology and Isfahan Pharmaceutical Research Center, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, IranMohammadiFereshtehKerman Pharmaceutics Research Center and Department of Pharmaceutics, Kerman University of Medical Sciences, Kerman, IranTaheritarighSadeghGenetics Division, Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, IranMohammadiZohreh 8 4 169 174 13 2 2016 20 4 2016

<p>Background: Antibiotic resistant bacteria can be considered as a main problem in infection management. Zinc oxide nanoparticles (ZnO NPs), individually or in combination with antibiotics, can be considered as good candidates for struggling against drug resistant bacteria.<br /> Methods: In this study, Zinc oxide nanoparticles were synthesized using sol-gel method in low temperature as a cost effective procedure and characterized by X-ray diffraction and Scanning Electron Microscopy. Antibacterial activity of 9 new combinations of Zinc oxide nanoparticles and ceftazidime was assessed against standards and new clinically isolated multi drug resistant <em>Pseudomonas aeruginosa (P. aeruginosa)</em>, in order to evaluate enhancement effect of synthesized Zinc oxide nanoparticles on antibacterial activity of ceftazidime.<br /> Results: The results indicated that desirable effects can be seen at 6 and 7 <em>mM</em> of Zinc oxide nanoparticles (60 to 100% inhibition). Moreover, after evaluation of 9 new combinations with various concentrations of both components, it was demonstrated that Zinc oxide nanoparticles can enhance the antibacterial activity of ceftazidime, against some bacterial strains of <em>P. aeruginosa</em>. The highest activity was observed with the concentration of 20 <em>&mu;g/ml</em> ceftazidime in the presence of 5, 6 or 7 <em>mM</em> of Zinc oxide nanoparticles.<br /> Conclusion: Zinc oxide nanoparticles in appropriate concentrations can be proposed as new and promising candidates for overcoming bacterial resistance.</p>