Avicenna J Med Biotech arij002 Avicenna Journal of Medical Biotechnology 2008-2835 2008-4625 Avicenna Research Institute ajmb10367 Immunogenicity of Cork and Loop Domains of Recombinant Baumannii acinetobactin Utilization Protein in Murine Model EsmaeilkhaniHamidRasooliIrajHashemiMasoomehNazarianShahramSefidFatemeh 11 2 180 186 31 10 2017 22 1 2018

<p>Background: <em>Acinetobacter baumannii (A. baumannii) </em>is a bothersome fatal patho-gen, particularly in healthcare system. Persistence and successful invasion of <em>A. baumannii </em>in vertebrate host cells largely depends on iron acquisition methods. Sidero-phore molecules and Iron-Regulated Outer Membrane Proteins (IROMPs) are the two essential members of iron acquisition system. Siderophores are secreted by bacteria to bind peripheral ferric iron and the IROMPs are expressed at the bacterial outer membrane as the receptor of ferric-siderophore complex. BauA is the corresponding siderophore receptor of <em>A. baumannii</em>. In this study, an attempt was made to assess the immunogenicity of antigenic domains of BauA which could be effective in iron uptake restriction and protection against bacterial invasion of the host cells.&nbsp;<br /> Methods: The antigenic domains of <em>bauA</em> were amplified from <em>A. baumannii </em>ATCC-19606. The PCR products were ligated into pET32a and expressed in<em> Escherichia coli (E. coli)</em> BL21 (DE3). Purification of recombinant domains was done by Nickel-Nitri-lotriacetic Acid (Ni-NTA) affinity chromatography. The recombinant domains were injected into BALB/C mice separately and in combination. Sero-reactivities of the recombinant proteins and mouse challenge tests were carried out.&nbsp;<br /> Results: The antibodies raised in mice could successfully recognize and bind antigenic domains. Passive immunization studies accomplished by immune rabbit serum inhibited the establishment of infection in mice.<br /> Conclusion: The results adapted from the present study disclose the protective role of functional domains of BauA, especially the cork domain, suggesting a novel recombinant immunogen candidate.</p>